a21209 goat anti dll4 r d systems (R&D Systems)

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R&D Systems a21209 goat anti dll4 r d systems
A21209 Goat Anti Dll4 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+dll4/Mouse+DLL4+Antibody/10__1016_slash_j__isci__2026__115205-188-47-50
Average 95 stars, based on 114 article reviews

a21209 goat anti dll4 r d systems - by Bioz Stars, 2026-09

95/100 stars

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Article Title: Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signaling.
Article Snippet: Primary antibodies used were rat anti-CD31 (BD Biosciences, Cat #553370, 1:250), rabbit anti-Ki67 (Abcam, Cat #ab16667, 1:500), rabbit anti-cleaved caspase-3 (Cell Signaling Technology, Cat #9664S, 1:500), Goat anti-DLL4 (R & D Systems, Cat #AF1389, 1:200), rabbit anti-Claudin-5 (ThermoFisher Scientific, Cat #34-1600, 1:500), rabbit anti-SOX9 (Millipore Sigma, Cat #ABE571, 1:500), mouse anti-Glutamine Synthetase (Millipore Sigma, Cat #MAB302, 1:500), rabbit anti-EAAT1 (Abcam, Cat #ab181036, 1:250), rabbit antiEAAT2 (Abcam, Cat #ab41621, 1:250), rabbit anti-GFAP (Millipore Sigma, Cat #AB5804, 1:500), rat anti-CD140a (BD Biosciences, Cat #558774, 1:100), rabbit anti-LEF1 (Cell Signaling Technology, Cat #2230s, 1:100), goat anti-PDGFRb (R & D Systems, Cat #AF1042, 1:250), rabbit anti-Laminin (Millipore Sigma, Cat #L9393, 1:500), rabbit anti-Aquaporin-4 (Millipore Sigma, Cat #A5971, 1:250), rabbit anti-Caveolin-1 (Abcam, Cat #ab18199, 1:500), rabbit anti-PLVAP (Proteintech, Cat #14452-1-AP, 1:250), mouse anti-NeuN (Millipore Sigma, Cat #MAB377, 1:500), rabbit anti-Calbindin (Millipore Sigma, Cat #AB1778, 1:250), mouse anti-PKCa (Santa Cruz Biotechnology, Cat #sc-8393, 1:1000), rabbit anti-Mfsd2a (a gift from Dr. Chenghua Gu, 1:300), goat anti-Podocalyxin (R & D Systems, Cat #AF1556-SP, 1:500), goat anti-ChAT (Novus Biologicals, Cat #NBP1-30052, 1:100), mouse anti-GAD67 (Millipore Sigma, Cat #MAB5406, 1:2000), rabbit anti-Tyrosine Hydroxylase (Abcam, Cat #ab6211, 1:1000), rabbit anti-Hexokinase II (Abcam, Cat #ab209847, 1:1000), rabbit anti-Pyruvate Kinase M2 (Cell Signaling Technology, Cat #4053, 1:1000), rabbit anti-VEGF-A (Millipore Sigma, Cat #SAB5700629, 1:2000), mouse anti-GAPDH (Proteintech, Cat #60004-1-Ig, 1:50000), FITC rat anti-mouse CD31 (BD Pharmingen, Cat #553372, 1:200), and APC rat anti-mouse CD31 (BD Pharmingen, Cat #551262, 1:200).

Article Title: Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodents
Article Snippet: Primary antibodies and concentrations used in this study were: goat anti-AP-2ε (*with antigen retrieval 1:500 and without antigen retrieval 1:200, AF5060, R&D Systems), chicken anti-GFP (1:3000, ab13970, Abcam), rabbit anti-GFP (1:1000, A-6455, Molecular Probes), *rabbit anti-Ki67 (1:1000, D3B5, Cell Signaling Technology), *mouse anti-Ki67 (1:500, 9449, Cell Signaling Technology), *mouse anti-Meis2 (1:500, sc-515470, Santa Cruz Biotechnology), rabbit anti-Meis2 (*with antigen retrieval 1:1000 and without antigen retrieval 1:500, ab73164, Abcam), *mouse anti-NeuroD1 (1:100, sc-46684, Santa Cruz Biotechnology), *goat anti-NeuroD1 (1:500, AF2746, R&D Systems), *rabbit anti-Notch1 (1:50, D1E11, Cell Signaling Technology), goat anti-Dll4 (*with antigen retrieval 1:50 and without antigen retrieval 1:25, AF1389, R&D Systems), *rabbit anti-NICD activated (1:75, D3B8, Cell Signaling Technology), *mouse anti-DsRd (1:500, TA180084, Origene), *rabbit anti-DsRd (1:500, 600-401-379, Rockland), HuC/D 8 μg/ml (Molecular Probes), rabbit anti-Foxn4 (1:50; ), *rabbit anti-Gαo1 (1:1000, PA5-59337, Invitrogen), *mouse anti-Gαi2 (1:250, clone L5, MAB3077, Millipore).

Article Title: Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signaling
Article Snippet: Primary antibodies used were rat anti-CD31 (BD Biosciences, Cat #553370, 1:250), rabbit anti-Ki67 (Abcam, Cat #ab16667, 1:500), rabbit anti-cleaved Caspase-3 (Cell Signaling Technology, Cat #9664S, 1:500), Goat anti-DLL4 (R & D Systems, Cat #AF1389, 1:200), rabbit anti-Claudin-5 (ThermoFisher Scientific, Cat #34-1600, 1:500), rabbit anti-SOX9 (Millipore Sigma, Cat #ABE571, 1:500), mouse anti- Glutamine Synthetase (Millipore Sigma, Cat #MAB302, 1:500), rabbit anti-EAAT1 (Abcam, Cat #ab181036, 1:250), rabbit anti-EAAT2 (Abcam, Cat #ab41621, 1:250), rabbit anti-GFAP (Millipore Sigma, Cat #AB5804, 1:500), rat anti-CD140a (BD Biosciences, Cat #558774, 1:100), rabbit anti-LEF1 (Cell Signaling Technology, Cat #2230s, 1:100), goat anti-PDGFRβ (R & D Systems, Cat #AF1042, 1:250), rabbit anti-Laminin (Millipore Sigma, Cat #L9393, 1:500), rabbit anti-Aquaporin-4 (Millipore Sigma, Cat #A5971, 1:250), rabbit anti-Caveolin-1 (Abcam, Cat #ab18199, 1:500), rabbit anti-PLVAP (Proteintech, Cat #14452-1-AP, 1:250), mouse anti-NeuN (Millipore Sigma, Cat #MAB377, 1:500), rabbit anti- Calbindin (Millipore Sigma, Cat #AB1778, 1:250), mouse anti-PKCα (Santa Cruz Biotechnology, Cat #sc- 8393, 1:1000), rabbit anti-Hexokinase II (Abcam, Cat #ab209847, 1:1000), rabbit anti-Pyruvate Kinase M2 (Cell Signaling Technology, Cat #4053, 1:1000), mouse anti-GAPDH (Proteintech, Cat #60004-1-Ig, 1:50000), FITC rat anti-mouse CD31 (BD Pharmingen, Cat #553372, 1:200), and APC rat anti-mouse CD31 (BD Pharmingen, Cat #551262, 1:200).

Article Title: Sex differences and risk factors for bleeding in Alagille syndrome.
Article Snippet: Primary antibodies used: rat anti-mouse CD31 (RRID: AB_394816, cat. #553370, BD Biosciences, 1:100), rabbit anti-ERG (RRID: AB_2630401, cat. #ab92513, Abcam, 1:200), goat anti-DLL4 (RRID: AB_354770, cat. #AF1389, RnD systems, dilution 1:2,000), goat anti-CD13 (RRID: AB_2227288, cat. #AF2335, RnD Systems, 1:200), goat anti-Jagged1 (RRID: AB_260348, cat. #J4127, Sigma-Aldrich, 1:500), mouse anti-human a-smooth muscle actin (Cy3 conjugated, RRID: AB_476856, cat. #C6198, Sigma Aldrich, 1:500; FITC conjugated, RRID: AB_476977, cat. #F3777, Sigma Aldrich, 1:500), rabbit anti-Myosin heavy chain 2022 The Authors EMBO Molecular Medicine 14: e15809 | 2022 15 of 23 D ow nloaded from https://w w w .em bopress.org on M ay 25, 2025 from IP 2405:acc0:1307:54c8:5ca0:9a5a:4252:4aae.

Data-independent acquisition:

Article Title:
Article Snippet: .. Primary antibodies used were: rat anti-CD31 (1:200, Dianova DIA-310), armenian hamster anti-CD31 (1:100, Abcam ab119341), biotinylated Isolectin B4 (1:200, Vector Laboratories B-1205), rat anti-Endomucin (1:50, Santa Cruz sc-65495), goat anti-DLL4 (1:50, R&D Systems AF1389), goat anti-EphB4 (1:50, R&D Systems AF446), mouse anti-Actin, α-Smooth Muscle - Cy3TM (1:200, Sigma-Aldrich C6198), mouse anti-Actin, α-Smooth Muscle (1:300, Sigma-Aldrich A5228), goat anti-SOX17 (1:300, R&D Systems AF1924), rabbit anti-Cardiac Troponin I (1:100, Abcam ab47003), rabbit anti-RFP (1:1000, Rockland 600-401-379, used to detect tdTomato), chicken anti-β-galactosidase (1:500, Abcam ab9361), rabbit antiMEF2A (1:100, Abcam ab109420), rabbit anti-MEF2C (1:1000, Cell Signalling 5030S) and rabbit anti-MEF2D (1:1000, Abcam ab32845). ..

Labeling:

Article Title: Sex differences and risk factors for bleeding in Alagille syndrome
Article Snippet: Retinas were flat-mounted in Vectashield (cat. no. H-1000, Vector Laboratories). .. Primary antibodies used: rat anti-mouse CD31 (RRID: AB_394816, cat. #553370, BD Biosciences, 1:100), rabbit anti-ERG (RRID: AB_2630401, cat. #ab92513, Abcam, 1:200), goat anti-DLL4 (RRID: AB_354770, cat. #AF1389, RnD systems, dilution 1:2,000), goat anti-CD13 (RRID: AB_2227288, cat. #AF2335, RnD Systems, 1:200), goat anti-Jagged1 (RRID: AB_260348, cat. #J4127, Sigma-Aldrich, 1:500), mouse anti-human a-smooth muscle actin (Cy3 conjugated, RRID: AB_476856, cat. #C6198, Sigma Aldrich, 1:500; FITC conjugated, RRID: AB_476977, cat. #F3777, Sigma Aldrich, 1:500), rabbit anti-Myosin heavy chain 2022 The Authors EMBO Molecular Medicine 14: e15809 | 2022 15 of 23 11 (RRID: AB_2147146, cat. #ab53219, Abcam, 1:200), goat antiCollagen I (RRID: AB_2753206, cat. #1310-01, Southern Biotech, 1:200), mouse anti-vimentin (RRID: AB_628437, cat. #sc6360, Santa Cruz, 1:200), rabbit anti-Transgelin (RRID: AB_443021, cat. #ab14106, Abcam, 1:200), rabbit anti-HISTONE H3 (phospho S10; RRID: AB_304763, cat. #ab5176, Abcam, 1:500), mouse anti-GFAP (Cy3 conjugated, RRID: AB_11213580, cat. # MAB3402C3, Millipore, 1:500), rabbit anti-Cleaved CASPASE3 (RRID: AB_2341188, cat. #9661 (D175), Cell Signaling, 1:700), rabbit anti-COLLAGEN type IV (RRID: AB_2276457, cat. # AB756P, Millipore, 1:500), mouse antiNeurofilament (RRID: AB_528399, cat. #RT97, DSHB, 1:200) nuclei were labeled with DAPI (cat. #D9542, Sigma Aldrich, 1:1,000). .. Images were captured using LSM 510 META, LSM 880, LSM980 (Carl Zeiss AG) microscopes and processed in Image J (NIH), and/ or Adobe image suite software (Adobe Inc).

Western Blot:

Article Title: Targeting FSCN1 with an oral small-molecule inhibitor for treating ocular neovascularization
Article Snippet: The membranes were then incubated with the corresponding primary and secondary antibodies, and the target protein bands were visualized using an Enhanced Chemiluminescence kit (Thermo Fisher Scientific, 32132). .. The following primary antibodies were used: rabbit anti-FSCN1 (Abcam, ab126772; IF 1:200, WB 1:1000, IP 1:100), mouse anti-GAPDH (Abcam, ab8245; WB 1:1000), mouse anti-F-actin (Abcam, ab205; WB 1:1000), rabbit anti-YAP (Cell Signaling Technology, 14074; IF 1:200, WB 1:1000), rabbit anti-p-YAP (Ser127) (Cell Signaling Technology, 4911; WB 1:1000), mouse anti-CDC42 (BD Biosciences, 610929; WB 1:500), rabbit anti-VEGFR2 (Cell Signaling Technology, 2479; WB 1:1000), rabbit anti-p-VEGFR2 (Tyr1175) (Cell Signaling Technology, 2478; WB 1:1000), goat anti-DLL4 (R&D Systems, AF1389; IF 1:200), and mouse anti-β‐actin (Millipore, A5441; WB 1:1000). .. The following secondary antibodies were used: goat anti-mouse HRP-conjugated antibodies (Beyotime, A0216; 1:1000) and goat anti-rabbit HRP-conjugated antibodies (Beyotime, A0208; 1:1000) were applied for WB, Alexa FluorTM Plus 488 Anti-mouse IgG (H + L) (Abcam, ab150077; IF 1:400), and DyLight 405-AffiniPure Donkey Anti-goat IgG (H + L) (Jackson ImmunoResearch,705–475-147,1:400) was applied for immunofluorescence.

Article Title: Targeting FSCN1 with an oral small-molecule inhibitor for treating ocular neovascularization.
Article Snippet: The membranes were then incubated with the corresponding primary and secondary antibodies, and the target protein bands were visualized using an Enhanced Chemiluminescence kit (Thermo Fisher Scientific, 32132). .. The following primary antibodies were used: rabbit antiFSCN1 (Abcam, ab126772; IF 1:200, WB 1:1000, IP 1:100), mouse anti-GAPDH (Abcam, ab8245; WB 1:1000), mouse anti-F-actin (Abcam, ab205; WB 1:1000), rabbit anti-YAP (Cell Signaling Technology, 14074; IF 1:200, WB 1:1000), rabbit anti-p-YAP (Ser127) (Cell Signaling Technology, 4911; WB 1:1000), mouse anti-CDC42 (BD Biosciences, 610929; WB 1:500), rabbit anti-VEGFR2 (Cell Signaling Technology, 2479; WB 1:1000), rabbit anti-p-VEGFR2 (Tyr1175) (Cell Signaling Technology, 2478; WB 1:1000), goat anti-DLL4 (R&D Systems, AF1389; IF 1:200), and mouse anti-β‐actin (Millipore, A5441; WB 1:1000). .. The following secondary antibodies were used: goat anti-mouse HRP-conjugated antibodies (Beyotime, A0216; 1:1000) and goat anti-rabbit HRP-conjugated antibodies (Beyotime, A0208; 1:1000) were applied for WB, Alexa FluorTM Plus 488 Anti-mouse IgG (H + L) (Abcam, ab150077; IF 1:400), and DyLight 405-AffiniPure Donkey Anti-goat IgG (H + L) (Jackson ImmunoResearch,705–475-147,1:400) was applied for immunofluorescence.

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